CLONING AND in silico STUDIES OF LIPASE GENE OF A THERMOPHILIC BACTERIA ISOLATED FROM THE HYDROTHERMAL VENT AREA OF KAWIO, NORTH SULAWESI

Underwater volcano is an example of a natural habitat thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus this research was to screen the bacterial isolates originally from for production thermostable lipase. In study, lipase gene selected isolate cloned, sequenced, and silico analysis performed predict characteristics protein. One three examined, showed positive reaction. Phylogenetic 16S rRNA that novel bacteria closely related Bacillus safensis strain FO-26b with 96,87% homology. Amino acid sequence similarity between pumilus B106 98% Further at protein level indicated isolated marine water member family I.4 ‘true lipase’ group.